Assays for Detecting Biological Superoxide

Authors

  • Hong Zhu Department of Cell Biology and Pathophysiology, Campbell University Jerry M. Wallace School of Osteopathic Medicine, P.O. Box 4280, Buies Creek, NC 27506, USA

Keywords:

5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide, Electron paramagnetic resonance, Ferricytochrone c reduction, Reactive oxygen species, Spin trapping, Superoxide, Superoxide dismutase

Abstract

Superoxide is produced from various endogenous and exogenous sources and has been shown to play an important role in diverse physiological and pathophysiological processes. Thus, detection and measurement of this ubiquitous reactive oxygen species (ROS) are instrumental in understanding its role in biology and medicine. This ROS protocol article describes two well-established assays for detecting superoxide, namely, superoxide dismutase-inhibitable ferricytochrome c reduction and electron paramagnetic resonance (EPR) spectrometry in conjunction with 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO)-spin trapping. The article provides a step-by-step, recipe-type protocol for each of the two assays and discusses their advantages and limitations.

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Published

2016-01-01

How to Cite

Zhu, H. (2016). Assays for Detecting Biological Superoxide. Reactive Oxygen Species, 1(1), 65–80. Retrieved from https://rosj.org/index.php/ros/article/view/20

Issue

Vol. 1 No. 1 (2016)

Section

ROS PROTOCOLS

License

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