Effect of Propofol and Fentanyl on Brain Oxidative Stress after Systemic Lipopolysaccharide Injection in Rats

Authors

  • Omar M.E. Abdel-Salam Department of Toxicology and Narcotics, National Research Centre, Cairo, Egypt
  • Eman R. Youness Department of Medical Biochemistry, National Research Centre, Cairo, Egypt
  • Nadia A. Mohammed Department of Medical Biochemistry, National Research Centre, Cairo, Egypt
  • Amr M.M. Ibrahim Department of Medical Biochemistry, National Research Centre, Cairo, Egypt

DOI:

https://doi.org/10.20455/ros.2021.r.801

Keywords:

Antioxidant; Fentanyl; Lipopolysaccharide; Neuronal injury; Nuclear factor kappaB; Oxidative stress; Propofol; Systemic endotoxemia

Abstract

Systemic inflammation causes brain oxidative stress, a prerequisite for neurodegeneration. In this study, we investigated the effect of the anesthetic agents propofol and fentanyl on brain oxidative stress during mild systemic endotoxemia induced by lipopolysaccharide (LPS) endotoxin. For this purpose, rats were administered LPS (400 μg/kg, intraperitoneally; i.p.), treated at the same time with different doses of propofol or fentanyl, i.p., and euthanized 4 h later. Other groups were treated with the saline, only propofol, or only fentanyl. Oxidative stress markers including malondialdehyde (MDA), nitric oxide (NO), and reduced glutathione (GSH) were determined. In addition, nuclear factor kappaB (NF-kB), paraoxonase-1 (PON-1), and butyrylcholinesterase (BChE) activities were measured in the brain tissue. Results showed that compared with the saline group, administration of LPS caused a marked and significant increase in brain MDA and NO combined with depletion of GSH and decreased PON-1 and BChE activities. Additionally, the active form of NF-kB was significantly increased in the brain of LPS only-treated rats. Treatment with propofol or fentanyl led to a marked and significant decrease in the levels of brain MDA and NO together with a significant increase in GSH and restoration of PON-1 and BChE activities. Furthermore, lower levels of active form of NF-kB were found following treatment with propofol or fentanyl compared with those in the LPS only group. Collectively, these results suggest that propofol and fentanyl exhibit an antioxidant action and attenuate the endotoxin-induced brain oxidative stress.

(First online: Feb 23, 2021)

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Published

2021-02-23

How to Cite

Abdel-Salam, O. M., Youness, E. R. ., Mohammed, N. A. ., & Ibrahim, A. M. . (2021). Effect of Propofol and Fentanyl on Brain Oxidative Stress after Systemic Lipopolysaccharide Injection in Rats. Reactive Oxygen Species, 11, r1–r13. https://doi.org/10.20455/ros.2021.r.801

Issue

Section

ORIGINAL RESEARCH